Composite

Part:BBa_K2152002:Design

Designed by: Shenjian Ai   Group: iGEM16_ShanghaiTechChina_B   (2016-10-13)


CsgA-EGF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 615
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use overlap PCR to connect CsgA and EGF with a linker. And to make a standard part, we performed site-directed mutagenesis to eliminate the restriction site of PstⅠ.


Source

For CsgA, it is from a gift from Allen Y. Chen, Biophysics Program, Harvard University. For EGF, it is synthesized by GenScript.

References